期刊
THERIOGENOLOGY
卷 133, 期 -, 页码 149-158出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2019.05.003
关键词
Melatonin; Pig; Oocyte-granulosa cell complexes; Preantral follicles; Developmental competence
资金
- Natural Science Project of Universities in Anhui Province [KJ2017A131, KJ2016A227]
- Anhui Provincial Key Foundation for Academic Talents of the University Discipline [gxbjZD2016023]
- Academic and Technical Talents and the Back-up Candidates Funding for Academic and Technical Talents and Backup Candidates Funding for Scientific Research Activities of Anhui Province [2016H093]
- Young Scholar Natural Science Foundation of Anhui Agricultural University [2016ZR020]
- Open Fund of Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding [AKLGRCB2017003]
- High-level Talent Research Foundation of Anhui Agricultural University [YJ2016-10]
- Anhui Provincial Natural Science Foundation [1708085QC55]
Melatonin has been reported to improve the survival rate of mouse and goat preantral follicles cultured in vitro. However, the role of melatonin in the development of oocyte-granulosa cell complexes (OGCs) isolated from preantral follicles remains unclear. Cumulus-oocyte complexes were isolated from OGCs cultured in vitro for 18.5 days and were then maturated in vitro for 42 h. The matured oocytes were parthenogenetically activated and were further cultured up to the blastocyst stage. We found that the developmental capacity of oocytes from in vitro cultured OGCs was significantly inferior to that from in vivo grown counterparts. Additionally, a 10(-5) M dose of melatonin added to the medium during in vitro culture of OGCs did not improve oocyte meiotic maturation but enhanced blastocyst rate of partheno-genetically activated embryos. Besides, these beneficial effects could be reversed by luzindole treatment, a melatonin membrane receptor antagonist. mRNA sequencing analysis further revealed that melatonin caused differential expression of 76 genes of which 75 were upregulated and 1 was downregulated in OGCs. Twelve of the 76 genes were identified as potential regulators of metabolic pathways by functional analysis. Taken together, these results indicate that melatonin improves developmental competence of porcine oocyte-granulosa cell complexes. (C) 2019 Published by Elsevier Inc.
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