Mapping quantitative trait loci for seed traits in Camelina sativa

Key message Genetic dissection of oil content and seed size in Camelina sativa was conducted by QTL mapping using a SNP-based linkage map and a recombinant inbred population. Camelina (Camelina sativa L. Crantz) is an oilseed crop that has great potential to provide sustainable feedstock for biofuel production and to improve dryland agriculture. A major breeding objective for camelina is to increase seed size and oil content. Understanding the genetics behind variations of seed size and associated traits such as oil content would help breeders develop varieties of increased oil yield that are more robust, easier to plant and harvest, and better for oil processing. In this study, we developed a recombinant inbred population derived from the two camelina accessions, Suneson and Pryzeth, with contrasting traits, especially seed size and oil content. Using 189 lines, a genetic map was constructed containing 2376 single nucleotide polymorphism markers spanning 2034.6 cM of 20 linkage groups with an average density of 1.5 cM per locus. Field trials were conducted for 2 years (2017 and 2018) in two environments (dryland and irrigated) in Bozeman, Montana. The results revealed important correlations of seed size with other associated traits such as oil content, pod size and seed number per pod. Significant QTLs were also discovered for these traits. The results of this study are the first step to isolate genes controlling seed development and oil accumulation and to develop advanced varieties of camelina better adapted to modern agriculture by marker-assisted breeding.