期刊
TALANTA
卷 199, 期 -, 页码 310-316出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.02.015
关键词
Janus Particles; OTA; Electrochemical aptasensor
资金
- National Key Research and Development Plan [2016YFF0203700]
- National Natural Science Foundation of China [51772289, 21575084]
- Shanxi Scholarship Council of China [2017-Key1]
- Shanxi Province Hundred Talents Project
In this study, a novel aptasensor based on Aptamer/NH2 Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 x 10(-5) nM to 10 nM, and the detection limit is 3.3 x 10(-3) pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.
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