期刊
TALANTA
卷 198, 期 -, 页码 71-77出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.01.078
关键词
Aflatoxin B1; AIEgens; Graphene Oxide; Aptamer; Label-free
资金
- National Natural Science Foundation of China [21705071, 21605074]
- Natural Science Foundation of Guangdong province of China [2016A030310362, 2014A030310274, 2017A030310604]
- Guangdong Province Office of Education Youth Innovation Talent Program [2016KQNCX096, 2017KTSCX118]
- Lingnan Normal University [ZL1611]
- Resource and Chemical Engineering Technology Research Center of Western Guangdong Province
The detection of Aflatoxin B1 (AFB1) has attracted extensive attention for food safety is a worldwide public health problem. Herein, a novel, simple and label-free fluorescent aptasensor, based on quaternized tetraphenylethene salt (TPE-Z), graphene oxide (GO) and AFB1 aptamer, has been constructed to detect AFBI. In the presence of AFB1, AFB1 aptamer undergoes a conformational switch from single stranded structure to the AFB1/AFB1 aptamer complex upon target binding, which induces the release of TPE-Z/AFB1 aptamer from the surface of GO. Thus, the fluorescence of TPE-Z/AFB1 aptamer is recovered. The assay can be performed by simply mixing TPE-Z, AFB1 aptamer, the GO and the AFB1 samples with a detection limit of 0.25 ng/mL. It is highly selective against other aflatoxins in foods and its performance has been verified in food samples (corn, milk and rice) with known concentration AFB1.
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