期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 216, 期 -, 页码 328-334出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.03.067
关键词
Ruthenium(II) complex; Phosphorescent probes; Biothiols; Cell imaging
类别
资金
- National Natural Science Foundation of China [21571137]
- Science Fund of Shandong Province for Excellent Young Scholars [ZR2017JL015]
- Natural Science Foundation of Shandong Province [ZR2015BL002]
Considering the excellent photochemical properties of ruthenium(II) complexes, two new ruthenium(II) complexes, RuL1-DNBS and RuL2-DNBS, have been developed as phosphorescence probes for detection of biothiols in 100:1 (v/v) Hepes buffer (20 mM, pH = 7.2)/CH3CN solution. The response rate was highly improved of these two probes toward biothiols because the steric interactions between 1H-imidazo [4, 5-f] [1,10] phenanthroline group and ortho-2, 4-dinitrobenzensulfonate resulted in a relatively rapid thiol-induced SNAr substitution reaction. RuL1-DNBS and RuL2-DNBS were weakly phosphorescent owing to the effectual photoinduced electron transfer from ruthenium(II) luminophore to the sensing group, 2,4-dinitrobenzenesulfonyl. After reacting with biothiols, the 2,4-dinitrobenzenesulfonyl group of RuL1-DNBS and RuL2-DNBS were cleavaged and the RuL1 and RuL2 were obtained. Meanwhile, the phosphorescence were turn-on. Both of these two probes can detect biothiols sensitively and selectively under physiological conditions with submicromolar detection limits. Furthermore, application of RuL2-DNBS for detecting of intracellular biothiols has been successfully performed in living Glioma cells. (C) 2019 Published by Elsevier B.V.
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