期刊
SMALL
卷 15, 期 48, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201902086
关键词
gold nanoparticles; ionic liquid; metal-organic frameworks; MoS2 nanosheets; photo-electrochemical immunosensing
类别
资金
- National Natural Science Foundation of China, China [21874157, 21675175, 21275166]
- Major Projects of Technical Innovation of Hubei Province, China [2017ACA172]
- Natural Science Foundation of Hubei Province, China [2018CFB617, 2015CFA092]
A hexafluorophosphate ionic liquid is used as a functional monomer to prepare a metal-organic framework (Zn-MOF). Zn-MOF is used as a template for MoS2 nanosheets synthesis and further carbonized to yield light-responsive ZnS/C/MoS2 nanocomposites. Zn-MOF, carbonized-Zn-MOF, and ZnS/C/MoS2 nanocomposites are characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction pattern, scanning electron microscopy (SEM), element mapping, Raman spectroscopy, X-ray photoelectron spectroscopy, fluorescence, and nitrogen-adsorption analysis. Carcinoembryonic antigen (CEA) is selected as a model to construct an immunosensing platform to evaluate the photo-electrochemical (PEC) performances of ZnS/C/MoS2 nanocomposites. A sandwich-type PEC immunosensor is fabricated by immobilizing CEA antibody (Ab(1)) onto the ZnS/C/MoS2/GCE surface, subsequently binding CEA and the alkaline phosphatase-gold nanoparticle labeled CEA antibody (ALP-Au-Ab(2)). The catalytic conversion of vitamin C magnesium phosphate produces ascorbic acid (AA). Upon being illuminated, AA can react with photogenerated holes from ZnS/C/MoS2 nanocomposites to generate a photocurrent for quantitative assay. Under optimized experimental conditions, the PEC immunosensor exhibits excellent analytical characteristics with a linear range from 2.0 pg mL(-1) to 10.0 ng mL(-1) and a detection limit of 1.30 pg mL(-1) (S/N = 3). The outstanding practicability of this PEC immunosensor is demonstrated by accurate assaying of CEA in clinical serum samples.
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