期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 290, 期 -, 页码 573-580出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.04.020
关键词
Colorimetry; Degradation; Manganite; Oxidase mimics; Test paper; Visual detection
资金
- National Key RAMP
- D Program of China [2018YFD0400600]
- Collaborative Innovation Major Special Projects of Guangzhou City [201604020007]
- National Natural Science Foundation of China [21675127]
- Fundamental Research Funds for the Central Universities [2018MS056, 2017MS075, 2014YB093, 2452015257]
- International and Hong Kong -Macau -Taiwan Collaborative Innovation Platform of Guangdong Province on Intelligent Food Quality Control and Process Technology Equipment [2015KGJHZ001]
- Guangdong Provincial RAMP
- D Centre for the Modern Agricultural Industry on Non-destructive Detection and Intensive Processing of Agricultural Products
- Common Technical Innovation Team of Guangdong Province on Preservation and Logistics of Agricultural Products [2016LM2154]
- Innovation Centre of Guangdong Province for Modern Agricultural Science and Technology on Intelligent Sensing and Precision Control of Agricultural Product Qualities
Despite the tremendous achievements of existing sensing techniques, sensitive and portable sensing of organophosphorus pesticides (OPs) in a non-polluting manner remains a challenging issue. This work proposed a facile colorimetric paper sensor using gamma-MnOOH nanowires (NWs) as a degradable nanozyme and 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic indicator for rapid and sensitive screening of OPs and acetylcholinesterase (AChE) activity. The sensing mechanism was based on the easy disintegration of the oxidase-like.-MnOOH NWs into invalid Mn2+ ions by the selective reaction with thiocholine (TCh) produced by AChE and acetylthiocholine iodide (ATCh), triggering a remarkable activity loss of MnOOH nanozymes towards TMB oxidation. The concentration of OPs, as an AChE inhibitor, was measured by the changes in absorbance at 652 nm or blue color of oxTMB products. Such detection platform in solution state achieved relatively low limits of detection (LODs) for AChE activity (0.007 mU mL(-1)), and for two typical OPs, i.e., omethoate (0.35 ng mL(-1)) and dichlorvos (0.14 ng mL(-1)), respectively. Further, the portable assembly of such AChE-nanozyme tandem reaction on test paper performed well, reaching LODs of 0.1 mU mL(-1) for AChE, 10 ng mL(-1) for omethoate, and 3 ng mL(-1) for dichlorvos, respectively. Meanwhile, this sensing system showed great selectivity and anti-interfering capacity in both solution and solid states, and worked well in real serum and vegetable samples. The abovementioned results, along with evidence of its good ecological sustainability, demonstrated the superiority of this well-performing platform for application in the food, environmental, and medical fields.
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