期刊
SCIENCE
卷 365, 期 6451, 页码 353-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaw5118
关键词
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资金
- National Natural Science Foundation of China [81820108016, 81741042, 31422031, 31725018, 31870817]
- Henan Provincial Obstetrical and Gynecological Diseases (Reproductive Medicine) Clinical Research Center
- National Key RD Program [2016YFC0900300, 2017YFA0102802]
- National Basic Research Program of China [2015CB856201]
- Beijing Municipal Science & Technology Commission [Z181100001318006]
- THU-PKU Center for Life Sciences
Histone modifications regulate gene expression and development. To address how they are reprogrammed in human early development, we investigated key histone marks in human oocytes and early embryos. Unlike that in mouse oocytes, the permissive mark trimethylated histone H3 lysine 4 (H3K4me3) largely exhibits canonical patterns at promoters in human oocytes. After fertilization, prezygotic genome activation (pre-ZGA) embryos acquire permissive chromatin and widespread H3K4me3 in CpG-rich regulatory regions. By contrast, the repressive mark H3K27me3 undergoes global depletion. CpG-rich regulatory regions then resolve to either active or repressed states upon ZGA, followed by subsequent restoration of H3K27me3 at developmental genes. Finally, by combining chromatin and transcriptome maps, we revealed transcription circuitry and asymmetric H3K27me3 patterning during early lineage specification. Collectively, our data unveil a priming phase connecting human parental-to-zygotic epigenetic transition.
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