期刊
SCIENCE
卷 365, 期 6456, 页码 919-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aav8907
关键词
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资金
- Agriculture and Food Research Initiative of the U.S. Department of Agriculture National Institute of Food and Agriculture [2015-67013-22811, 2018-67013-27425]
- Purdue AgSEED program
- North Central Soybean Research Program
- Indiana Soybean Alliance
Rhizobial infection and root nodule formation in legumes require recognition of signal molecules produced by the bacteria and their hosts. Here, we show that rhizobial transfer RNA (tRNA)-derived small RNA fragments (tRFs) are signal molecules that modulate host nodulation. Three families of rhizobial tRFs were confirmed to regulate host genes associated with nodule initiation and development through hijacking the host RNA-interference machinery that involves ARGONAUTE 1. Silencing individual tRFs with the use of short tandem target mimics or by overexpressing their targets represses root hair curling and nodule formation, whereas repressing these targets with artificial microRNAs identical to the respective tRFs or mutating these targets with CRISPR-Cas9 promotes nodulation. Our findings thus uncover a bacterial small RNA-mediated mechanism for prokaryote-eukaryote interaction and may pave the way for enhancing nodulation efficiency in legumes.
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