4.5 Article

microRNA-14 as an efficient suppressor to switch off ecdysone production after ecdysis in insects

期刊

RNA BIOLOGY
卷 16, 期 9, 页码 1313-1325

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2019.1629768

关键词

Microrna; molting timing; developmental synchrony; ecdysone

资金

  1. National Key Research and Development Program [2017YFD0200900, 2016YFC1200600]
  2. National Natural Science Foundation of China (NSFC) [31772238]
  3. NSFC [31760514]

向作者/读者索取更多资源

The precise increase and decrease of hormone ecdysone are critical for accurate development in insects. Most previous works focus on transcriptional activation of ecdysone production; however, little is known about the mechanism of switching off ecdysone biosynthesis after ecdysis. Here, we showed that the precursor microRNA-14 (pre-miR-14) encodes two mature miRNAs in silkworm; both of these two mature miRNAs regulate various genes in the ecdysone-signalling pathway. Bmo-miR-14-5p targets on nine genes whereas Bmo-miR-14-3p targets on two genes in the same pathway. These two mature miRNAs increased immediately after the ecdysis, efficiently suppressing the 20-hydroxyecdysone (20E) biosynthesis, the upstream regulation, and the downstream response genes. Knocking down either of two mature miRNAs or both of them delays moult development, impairing development synchrony in antagomir-treated groups. In addition, overexpressing Bmo-miR-14-5p but not Bmo-miR-14-3p significantly affected the 20E titer and increased the moulting time variation, suggesting that Bmo-miR-14-5p, though it is less abundant, has more potent effects in development regulation than Bmo-miR-14-3p. In summary, we present evidence that a pre-miRNA encodes two mature miRNAs targeting on the same pathway, which significantly improves miRNA regulation efficiencies to programmatically switch off ecdysone biosynthesis.

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