期刊
RNA BIOLOGY
卷 16, 期 10, 页码 1401-1413出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2019.1631643
关键词
Adipocyte differentiation; clonal expansion; long non-coding RNA; DNMT1; methylation regulation
资金
- National High-tech R&D Program of China [2014AA021103]
- National Key Basic Research Program of China [2012CB518000]
- National Basic Research Program of China [2011CBA01102]
- National Natural Science Foundation of China [31571403]
- Beijing Natural Science Foundation [5132015]
Adipocyte differentiation is a coordinated cellular process, which involves a series of dynamic molecular events. Up-regulation of long noncoding RNA slincRAD expression was found to occur in the early differentiation stages of 3T3-L1 cell, prior to the regulation of major transcription factors. By interacting with DNMT1 in S phase, slincRAD guides this essentially epigenetic factor to mediate promoter methylation of a batch of cell cycle-related genes, including cyclin-dependent kinase inhibitor p21. The regulation promotes the growth-arrested cells to re-enter into cell cycle under hormone induction and thereby advances the process of differentiation to clonal expansion stage. The abolishment of the interaction between slincRAD and DNMT1 by slincRAD knockdown results in a defective epigenetic regulation and finally compromised adipogenesis. Collectively, our study characterizes the epigenetic regulation of lncRNA involved in the early stage of adipogenesis.
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