期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 29, 页码 14761-14768出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1902087116
关键词
serotonin transporter polymorphism; insula cortex; serotonin 2A receptor; anxiety
资金
- Medical Research Council (MRC) Programme Grant [MR/M023990/1]
- Behavioural and Clinical Neuroscience Institute - Wellcome Trust
- Behavioural and Clinical Neuroscience Institute - MRC
- MRC [G0900903]
- MRC [G0900903, G1100307, MR/M023990/1] Funding Source: UKRI
Genetic variation in the serotonin transporter gene (SLC6A4) is associated with vulnerability to affective disorders and pharmacotherapy efficacy. We recently identified sequence polymorphisms in the common marmoset SLC6A4 repeat region (AC/C/G and CT/T/C) associated with individual differences in anxiety-like trait, gene expression, and response to antidepressants. The mechanisms underlying the effects of these polymorphisms are unknown, but a key mediator of serotonin action is the serotonin 2A receptor (5HT(2A)). Thus, we correlated 5HT(2A) binding potential (BP) and RNA gene expression in 16 SLC6A4 genotyped marmosets with responsivity to 5HT(2A) antagonism during the human intruder test of anxiety. Voxel-based analysis and RNA measurements showed a reduction in 5HT(2A) BP and gene expression specifically in the right posterior insula of individuals homozygous for the anxiety-related variant AC/C/G. These same marmosets displayed an anxiogenic, dose-dependent response to the human intruder after 5HT(2A) pharmacological antagonism, while CT/T/C individuals showed no effect. A voxel-based correlation analysis, independent of SLC6A4 genotype, revealed that 5HT(2A) BP in the adjacent right anterior insula and insula proisocortex was negatively correlated with trait anxiety scores. Moreover, 5HT(2A) BP in both regions was a good predictor of the size and direction of the acute emotional response to the human intruder threat after 5HT(2A) antagonism. Our findings suggest that genetic variation in the SLC6A4 repeat region may contribute to the trait anxious phenotype via neurochemical changes in brain areas implicated in interoceptive and emotional processing, with a critical role for the right insula 5HT(2A) in the regulation of affective responses to threat.
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