期刊
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
卷 49, 期 9, 页码 927-934出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2019.1643735
关键词
Corn husk; CaCO3; FEG-SEM; lignocellulosic enzymes; Sphingobacterium sp; ksn
类别
资金
- DBT, New Delhi India [BT/PR/10397/PBD/26/402/2013]
The present work was aimed at studying the production of lignocellulolytic enzymes, namely cellulase, xylanase, pectinase, mannanase, and laccase by a newly isolated bacterium Sphingobacterium sp. ksn-11, utilizing various agro-residues as a substrate under submerged conditions. The production of lignocellulolytic enzymes was found to be maximum at the loading of 10%(w/v) agro-residues. The enzyme secretion was enhanced by two-fold at 2 mM CaCO3, optimum pH 7, and temperature 40 degrees. The Field Emission Gun-Scanning Electron Microscope (FEG-SEM) results have shown the degradative effect of lignocellulases; cellulase, xylanase, mannanase, pectinase, and laccase on corn husk with 3.55 U/ml, 79.22 U/ml, 12.43 U/ml, 64.66 U/ml, and 21.12 U/ml of activity, respectively. The hydrolyzed corn husk found to be good adsorbent for polyphenols released during hydrolysis of corn husk providing suitable conditions for stability of lignocellulases. Sphingobacterium sp. ksn is proved to be a promising candidate for lignocellulolytic enzymes in view of demand for enzymes in the biofuel industry.
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