期刊
POULTRY SCIENCE
卷 98, 期 11, 页码 5321-5329出版社
OXFORD UNIV PRESS
DOI: 10.3382/ps/pez319
关键词
avian reovirus; isolation and identification; pathogenicity; chicken
资金
- funds for Research and Development from Tianjin Ruipu Biotechnology Co. Ltd., China
- National Natural Science Foundation of China [31672520]
- National Natural Science Foundation Youth Funding Project of China [31802157, 31602027]
- Postdoctoral Science Foundation of the Jiangsu Province [2018K206C]
In recent years, emerging avian reovirus (ARV) strains causing viral arthritis have become a challenge to the worldwide chicken industry, and were responsible for significant economic losses. In this study, we characterized emerging variant ARV strains and examined their genetic relationship and pathogenicity variation with reference strains. A total of 18 emerging variant ARV strains were isolated from tendon and capsular synovial fluid of broiler chickens with clinical cases of arthritis/tenosynovitis at commercial farms in China. Comparative analysis based on sigma C sequence showed that 4/18 isolates were in the same cluster (Cluster 1) as vaccine strains (S1133), whereas 14 of 18 isolates were in Clusters 2, 3, and 6. The field isolates shared a rather low identity (38.1 to 81.9%) with S1133 in Cluster 1, especially for those from Cluster 6 (38.1 to 67.2%). A higher ARV isolation rate was observed in chicken embryos (47/61) compared to cell culture (37/61) through PCR with a detection primer. A total of 3 isolates were selected to infect specific-pathogen-free (SPF) chickens, showing that the tested isolates, especially that from Cluster 6, displayed greater pathogenicity than S1133 strain, characterized by higher incidence. These findings suggest that the virulence of Chinese ARVs has been increasing rapidly in recent years, and the vaccine need to be updated correspondingly.
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