期刊
AQUATIC TOXICOLOGY
卷 175, 期 -, 页码 106-116出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2016.03.013
关键词
Novel microRNA; Marine medaka; Genome sequencing; Small RNA sequencing; Biomarkers
资金
- State Key Laboratory in Marine Pollution (SKLMP) Seed Collaborative Research Fund [PJ: SKLMP/SCRF/0013]
- Lo Kwee-Seong Biomedical Research Fund
- Lee Hysan Foundation
The marine medaka Oryzias melastigma has often been used as a marine fish model to investigate the biological responses to environmental stresses and pollutants in marine environments. miRNAs are post transcriptional regulators of many biological processes in a variety of organisms, and have been shown to be affected by environmental stresses, but the novel miRNA profile of marine medaka has not been reported. Using both genome and small RNA sequencings coupled with different bioinformatics analyses, we have discovered 58, 82, 234, and 201 unannotated miRNAs in the brain, liver, ovary and testis tissues of marine medaka, respectively. Furthermore, these novel miRNAs were found to target genes with tissue-specific roles such as neuron development and synaptic transmission in the brain, glucose and fat metabolism in the liver and steroidogenesis in the gonads. We here report, for the first time, novel miRNA profile of marine medaka, which will provide a foundation for future biomarkers and transgenerational studies for the assessment of environmental stresses and pollutions in the marine environments. In a boarder context, our data will provide novel insight into our knowledge of miRNome and miR research. (C) 2016 Elsevier B.V. All rights reserved.
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