期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 18, 期 1, 页码 20-31出版社
WILEY
DOI: 10.1111/pbi.13225
关键词
adenine; base editors; cytidine; CRISPR; Cas9; crop improvement; genome editing
资金
- China Priority Program-Breeding of Seven Major Crops [2017YFD01100100]
- Chinese Academy of Agricultural Sciences
- Talented Young Scientist Program of China
Targeted mutagenesis via genome-editing technologies holds great promise in developing improved crop varieties to meet future demands. Point mutations or single nucleotide polymorphisms often determine important agronomic traits of crops. Genome-editing-based single-base changes could generate elite trait variants in crop plants which help in accelerating crop improvement. Among the genome-editing technologies, base editing has emerged as a novel and efficient genome-editing approach which enables direct and irreversible conversion of one target base into another in a programmable manner. A base editor is a fusion of catalytically inactive CRISPR-Cas9 domain (Cas9 variants) and cytosine or adenosine deaminase domain that introduces desired point mutations in the target region enabling precise editing of genomes. In the present review, we have summarized the development of different base-editing platforms. Then, we have focussed on the current advances and the potential applications of this precise technology in crop improvement. The review also sheds light on the limitations associated with this technology. Finally, the future perspectives of this emerging technology towards crop improvement have been highlighted.
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