Metabolomic variability of four macroalgal species of the genus Lobophora using diverse approaches

Among comparative metabolomic studies used in marine sciences, only few of them are dedicated to macroalgae despite their ecological importance in marine ecosystems. Therefore, experimental data are needed to assess the scopes and limitations of different metabolomic techniques applied to macroalgal models. Species of the genus Lobophora belong to marine brown algae (Family: Dictyotaceae) and are widely distributed, especially in tropical coral reefs. The species richness of this genus has only been unveiled recently and it includes species of diverse morphologies and habitats, with some species interacting with corals. This study aims to assess the potential of different metabolomic fingerprinting approaches in the discrimination of four well known Lobophora species (L. rosacea, L. sonderii, L. obscura and L. monticola). These species present distinct morphologies and are found in various habitats in the New Caledonian lagoon (South-Western Pacific). We compared and combined different untargeted metabolomic techniques: liquid chromatography-mass spectrometry (LC-MS), nuclear magnetic resonance (H-1-NMR) and gas chromatography (GC-MS). Metabolomic separations were observed between each Lobophora species, with significant differences according to the techniques used. LC-MS was the best approach for metabotype distinction but a combination of approaches was also useful and allowed identification of chemomarkers for some species. These comparisons provide important data on the use of metabolomic approaches in the Lobophora genus and will pave the way for further studies on the sources of metabolomic variations for this ecologically important macroalgae.