期刊
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
卷 24, 期 8, 页码 927-934出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10837450.2018.1556690
关键词
Polymeric micelles; silymarin; renoprotective effect; cisplatin; chitosan
资金
- Thailand Research Funds through the Golden Jubilee Ph.D. Program [PHD/0139/2557]
- Thailand Research Funds through the Research Team Promotion Grant [RTA6180003]
- Silpakorn University [SURDI610110]
- Faculty of Pharmacy, Silpakorn University
This research aimed to evaluate the effects of silymarin (SM)-loaded polymeric micelles (PMs) on the renal toxicity and anticancer activity of cisplatin. Amphiphilic chitosan derivatives were employed to develop SM-loaded PMs. The permeation across an intestinal membrane, cytotoxicity, and renal toxicity of cisplatin during the treatment were evaluated. The SM-loaded PMs had small particle sizes (326-336 nm), negative surface charge, high entrapment efficiency (47-70%), and demonstrated pH-sensitive release. Rapid drug release was obtained at pH 7.4 (81-87% in 4 h). The SM-loaded PMs exhibited higher flux than free SM. Moreover, the pretreatment of SM (50-100 mu g/mL)-loaded PMs increased the killing efficacy of cisplatin on the cancer cells. The renoprotective effect was witnessed (p < 0.05) on the cells pretreated with SM-loaded benzyl-functionalized succinyl chitosan (BSC) PMs compared with those treated with only cisplatin, which the % cell viability increased from 29% to 82% and 96% for the PMs with SM concentration of 50 and 100 mu g/mL, respectively. Moreover, the reduction in cell apoptosis and necrosis induced by cisplatin has been observed. In conclusion, SM-loaded BSC PMs could improve the bioavailability of SM, enhance the therapeutic effect, and protect renal damage during the treatment with cisplatin.
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