期刊
OSTEOARTHRITIS AND CARTILAGE
卷 27, 期 10, 页码 1537-1544出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2019.05.022
关键词
Verteporfin; Photochemical bonding; Articular cartilage; Focal defect repair; Osteochondral graft; Allograft
资金
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [R21 AR063876]
- Stanford Bio-X Interdisciplinary Initiatives Program
- National Science Foundation Graduate Research Fellowship Program
- Ford Foundation Predoctoral Fellowship
Objective: To evaluate the effectiveness of verteporfin as a photosensitizer to photochemically bond articular cartilage tissues and determine bond durability in vitro. Design: Bond strength induced by verteporfin over a range of concentrations and light exposure conditions was investigated using a disk-annulus model and a pushout test. Exposure was parameterized by varying either irradiance or irradiation time. Bond robustness in a cell-mediated degeneration environment was examined by exposing newly bonded samples to interleukin-1 alpha for the first 4 days of a 7-day culture period, followed by mechanical testing and biochemical and cellular viability assays. Results: Photochemical bonding using verteporfin produced high bonding shear strengths at relatively low photosensitizer concentrations. Low exposures produced by either low irradiance or short irradiation time were sufficient to produce shear strengths comparable to those previously produced with phthalocyanine photosensitizers with substantially higher light exposure. Photochemically produced bonds were resistant to cell-mediated degeneration in vitro with no evident differences in cell viability among treatments. Conclusions: Verteporfin offers distinct advantages as a photosensitizer for photochemical bonding of articular cartilage due to the production of strong, durable bonds at relatively low light exposures. Further exploration may lead to clinically feasible strategies to augment cartilage repair techniques. (C) 2019 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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