期刊
NEUROSCIENCE RESEARCH
卷 155, 期 -, 页码 27-33出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neures.2019.07.001
关键词
Drug delivery system; Inner ear; Luciferin; Bioluminescence imaging; Mouse
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) [15K10762]
- Brain/MINDS from Japan Agency for Medical Research and Development (AMED)
- Grants-in-Aid for Scientific Research [15K10762] Funding Source: KAKEN
Combination of systemic and local drug therapy has been proved more effective and safer for idiopathic sudden sensorineural hearing loss by some clinical trials, and there are few laboratory researches on its pharmacokinetic behaviors in the inner ear. In the present study, we use a new in vivo imaging system to compare the pharmacokinetics of combined therapy (CT), with intravenous (IV) or transtympanic (TT) injection alone in both ears of transgenic GFAP-Luc mice. Biological half-life, total photon counts and the area under the curve (AUC) value significantly increased after CT. However, adding IV to TT injection cannot strengthen the peak photon of the drug in the inner ear. In addition, when D-luciferin is injected to the left ear the volume of total photon count and AUC value of CT-left ear are larger than the combined volume of Tr-left ear and IV-left ear, suggesting a synergistic effect, and those of CT-right ear are almost equal to the summation of those of IV-right group and TT-right group, suggesting no amplifying effect on the risk of systemic side effect. This study showed that CT could deliver more drugs into the inner ear, and brought a longer therapeutic window, and were more effective than intravenous or transtympanic injection alone in the pharmacokinetics. (C) 2019 Published by Elsevier B.V.
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