期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 26, 期 7, 页码 571-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41594-019-0241-y
关键词
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资金
- National Key R&D Program of China [2018YFC1004500]
- INSERM
- CEA
- CNRS
- fondation France Alzheimer (AAP Sciences Medicales 2018)
- Swiss National Science Foundation [31003A_166608]
- Shenzhen Government [Y01226136]
- Thousand Young Talents Program
- University Grenoble Alpes
- La Ligue Contre le Cancer comite de l'Isere
- PIC-GIN (Photonic Imaging Center of GIN, University Grenoble Alpes-Inserm) [U1216]
Vasohibins are tubulin tyrosine carboxypeptidases that are important in neuron physiology. We examined the crystal structures of human vasohibin 1 and 2 in complex with small vasohibin-binding protein (SVBP) in the absence and presence of different inhibitors and a C-terminal alpha-tubulin peptide. In combination with functional data, we propose that SVBP acts as an activator of vasohibins. An extended groove and a distinctive surface residue patch of vasohibins define the specific determinants for recognizing and cleaving the C-terminal tyrosine of alpha-tubulin and for binding microtubules, respectively. The vasohibin-SVBP interaction and the ability of the enzyme complex to associate with microtubules regulate axon specification of neurons. Our results define the structural basis of tubulin detyrosination by vasohibins and show the relevance of this process for neuronal development. Our findings offer a unique platform for developing drugs against human conditions with abnormal tubulin tyrosination levels, such as cancer, heart defects and possibly brain disorders.
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