期刊
NATURE MATERIALS
卷 18, 期 10, 页码 1071-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41563-019-0391-7
关键词
-
类别
资金
- NIH Director's New Innovator Award Program [1DP2-EB018657]
- Rowland Fellows Program
- Wyss Institute for Biologically Inspired Engineering at Harvard University
Cell stiffness measurements have led to insights into various physiological and pathological processes. Although many cellular behaviours are influenced by intracellular mechanical forces that also alter the material properties of the cell, the precise mechanistic relationship between intracellular forces and cell stiffness remains unclear. Here we develop a cell mechanical imaging platform with high spatial resolution that reveals the existence of nanoscale stiffness patterns governed by intracellular forces. On the basis of these findings, we develop and validate a cellular mechanical model that quantitatively relates cell stiffness to intracellular forces. This allows us to determine the magnitude of tension within actin bundles, cell cortex and plasma membrane from the cell stiffness patterns across individual cells. These results expand our knowledge on the mechanical interaction between cells and their environments, and offer an alternative approach to determine physiologically relevant intracellular forces from high-resolution cell stiffness images.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据