期刊
MOLECULES
卷 24, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/molecules24152748
关键词
Aristoteline; vascular activity; calcium channels; potassium channels; rat
资金
- Network for Extreme Environments Research project (NEXER) (Universidad de Antofagasta, Chile) [ANT1756]
- Universidad de La Frontera (Temuco, Chile), CONICYT [21151406]
Alkaloids derived from plants have shown great medicinal benefits, and are often reported for their use in cardiovascular disease management. Aristotelia chilensis (Molina) Stuntz (Maqui) has shown important medicinal properties in traditional useage. In this study, we evaluated the effect of the indole-alkaloid aristoteline (ARI), isolated from leaves of Maqui, on vascular reactivity of isolated aortic rings from normotensive rats. ARI induced relaxation (100%) in a concentration-dependent manner in intact or denuded-endothelium aortic rings pre-contracted with phenylephrine (PE; 1 mu M). However, a specific soluble guanylyl cyclase inhibitor (ODQ; 1 mu M) significantly reduced the relaxation to ARI in aortic rings pre-contracted with PE. In the presence of ARI, the contraction induced by KCl or PE was significantly (p < 0.05) decreased. Interestingly, the potassium channel blockade with 10 mu M BaCl2 (Kir), 10 mu M glibenclamide (K-ATP), 1 mM tetraethylammonium (TEA; KCa1.1), or 1 mM 4-aminopyridine (4-AP; Kv) significantly (p < 0.05) reduced the ARI-induced relaxation. ARI significantly (p < 0.05) reduced the contractile response to agonist of Ca(V)1.2 channels (Bay K8644; 10 nM), likely reducing the influx of extracellular calcium through plasma membrane. The mechanisms associated with this process suggest an activation of the potassium channels, a calcium-induced antagonism and endothelium independent vasodilation that possibly involves the nitric oxide-independent soluble guanylate cyclase pathway.
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