期刊
MOLECULES
卷 24, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/molecules24142641
关键词
TMT; proteomics; grouper fillets; quality deterioration; refrigerated storage
资金
- National Natural Science Foundation of China [31571914]
- China Agriculture Research System [CARS-47]
- Construction Project of Public Service Platform for Shanghai Municipal Science and Technology Commission [17DZ2293400]
- Shanghai Municipal Science and Technology Project to enhance the capabilities of the platform [19DZ2284000]
A TMT (Tandem Mass Tag)-based strategy was applied to elucidate proteins that change in proteomes of grouper fillets during refrigerated storage. In addition, quality analyses on pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) for grouper fillets were performed. A total of 64 differentially significant expressed proteins (DSEPs) were found in the results in the Day 0 vs. Day 6 group comparison and the Day 0 vs. Day 12 group comparison. It is worth mentioning that more proteome changes were found in the Day 0 vs. Day 12 comparisons. Bioinformatics was utilized to analyze the DSEP. UniProt Knowledgebase (UniProtKB), Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein interaction network analysis were adopted. All DSEPs were classified into seven areas by function: binding proteins, calcium handling, enzymes, heat shock protein, protein turnover, structural proteins and miscellaneous. The numbers of proteins that correlated closely with pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) were 4, 3, 6 and 8, respectively.
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