期刊
MOLECULAR CELL
卷 75, 期 5, 页码 933-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2019.06.013
关键词
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资金
- National Institute of General Medical Sciences from the National Institutes of Health [P30 GM124165]
- NIH-ORIP HEI grant [S10 RR029205]
- DOE Office of Science [DE-AC02-06CH11357]
- Geoffrey Beene Cancer Research Center [GM129430]
- Memorial Sloan Kettering Cancer Center Core Grant [P30CA008748]
- IFM Therapeutics, LLC
Target RNA binding to crRNA-bound type III-A CRISPR-Cas multi-subunit Csm surveillance complexes activates cyclic-oligoadenylate (cA(n)) formation from ATP subunits positioned within the composite pair of Palm domain pockets of the Csm1 subunit. The generated cA(n) second messenger in turn targets the CARF domain of trans-acting RNase Csm6, triggering its HEPN domain-based RNase activity. We have undertaken cryo-EM studies on multi-subunit Thermococcus on-nurineus Csm effector ternary complexes, as well as X-ray studies on Csm1-Csm4 cassette, both bound to substrate (AMPPNP), intermediates (pppA(n)), and products (cA(n)), to decipher mechanistic aspects of cA(n) formation and release. A network of intermolecular hydrogen bond alignments accounts for the observed adenosine specificity, with ligand positioning dictating formation of linear pppA(n) intermediates and subsequent cA(n) formation by cyclization. We combine our structural results with published functional studies to highlight mechanistic insights into the role of the Csm effector complex in mediating the cA(n) signaling pathway.
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