期刊
MICROCHIMICA ACTA
卷 186, 期 6, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3485-7
关键词
Organophosphates; Carbamates; Platinum nanoparticles; Enzyme inhibition; Colorimetric assay; Enzyme mimic
资金
- National Natural Science Foundation of China [31501571, 31772071]
- China Agriculture Research System [CARS-05-05A-03]
- Guangxi Innovation-driven Development Project [AA17204043-2]
- Key Research and Development Program of Hebei [17274802D]
A novel and highly sensitive enzyme inhibition assay was developed for the rapid detection of the organophosphate pesticide dichlorvos and the carbamate pesticide carbofuran. It achieves signal amplification by the secondary catalysis of platinum nanoparticles. Acetylcholinesterase (AChE) is capable of catalyzing the hydrolysis of acetylthiocholine to form thiocholine. Thiocholine causes the aggregation of citrate-capped platinum nanoparticles which then lose their peroxidase-mimicking properties. After addition of pesticides, the activity of AChE is inhibited, less thiocholine is produced, less aggregation occurs, and the peroxidase-mimetic properties are increasingly retained. In the presence of tetramethylbenzidine and H2O2, a deep blue coloration with an absorption maximum at 650 nm will be formed. The assay was applied to the determination of dichlorvos and carbofuran, and detection limits of 2.3 mu g.L-1 and 1.4 mu g.L-1 were obtained, respectively. Recovery experiments with spiked tap water and pears gave satisfactory relative standard deviations.
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