期刊
MICROCHIMICA ACTA
卷 186, 期 8, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3613-4
关键词
MnO2 nanosheets; Dual-readout; Dual-component; Colorimetric and fluorometric method; GSH and Ag+
资金
- National Natural Science Foundation of China [21645008, 21305042]
- Scientific Research Fund of Hunan Provincial Education Department [18A010, 17C0947]
- Science and Technology Department [14JJ4030]
- construct program of the key discipline in Hunan province
- Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
A fluorimetric and colorimetric method is described for the determination of glutathione (GSH) and silver (I). It is based on the use of MnO2 nanosheets that were prepared by solution mixing and exfoliation. They display oxidase-mimicking activity and can catalyze the oxidation of o-phenylenediamine (OPD) to form yellow 2,3-diaminophenazine (DAP) with an absorption maximum at 410nm. DAP also has a yellow fluorescence (with a peak at 560nm). The MnO2 nanosheets can be rapidly reduced to Mn2+ by GSH. This reduces the efficiency of the oxidase mimic MnO2 and causes a decrease in fluorescence and absorbance intensity. However, on addition of Ag+, a complex is formed with GSH. It prevents the destruction of MnO2 nanosheets so that the enzyme mimicking activity is retained. A dual-method for the determination of GSH and Ag(I) was developed. It has excellent sensitivity for GSH with lower detection limits of 62nM (fluorimetric) and 0.94 mu M (colorimetric). The respective data for Ag(I) are 70nM and 1.15 mu M. The assay was successfully applied to the determination of GSH and Ag(I) in spiked serum samples.
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