期刊
METHODS
卷 168, 期 -, 页码 76-83出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2019.07.007
关键词
Carbon dots; Roast duck; Formation; Fluorescent probes; Bio-imaging
资金
- National Key Research and Development Project of China [2018YFD0901106, 2017YFD0400103]
- National Natural Science Foundation of China [31872915, 31601389]
Biocompatible fluorescent carbon dots (CDs) were prepared via a simple and green route using duck breasts as a natural carbon source. The CDs from duck breasts were well dispersed, and their mean particle size decreased from 2.59 to 1.95 nm when the roasting temperature increased from 200 to 300 degrees C. Abundant functional groups such as -OH, -COOH, and -NH2 were observed on the surface of the CDs, providing the CDs with good water solubility. These CDs emitted strong fluorescence under ultraviolet light irradiation and exhibited superior photostability. The absolute fluorescence quantum yield of CDs rose from 10.53% to 38.05% when the relative nitrogen content of CDs increased from 7.18% to 12.73%. The CDs showed low toxicity to PC12 cells for prolonged exposure. Therefore, the duck CDs were successfully developed as fluorescent probes for in vitro PC12 cells and in vivo Caenorhabditis elegans imaging. These results indicated that the CDs derived from roast duck were biocompatible and can potentially be used as probes in bio-imaging.
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