期刊
MARINE DRUGS
卷 17, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/md17060355
关键词
rhamnose-binding protein; anti-biofilm; quorum sensing factor; anti-infection
资金
- Ministry of Science and Technology (MOST) [MOST 103-2627-M-007-006, MOST 107-0210-01-19-04]
- Simpson Biotech Co., Ltd. (Taiwan)
- EU
- European Regional Development Fund [GINOP-2.3.2-15-2016-00008]
- New National Excellence Program of the Ministry of Human Capacities of Hungary [UNKP-18-3]
More than 80% of infectious bacteria form biofilm, which is a bacterial cell community surrounded by secreted polysaccharides, proteins and glycolipids. Such bacterial superstructure increases resistance to antimicrobials and host defenses. Thus, to control these biofilm-forming pathogenic bacteria requires antimicrobial agents with novel mechanisms or properties. Pseudomonas aeruginosa, a Gram-negative opportunistic nosocomial pathogen, is a model strain to study biofilm development and correlation between biofilm formation and infection. In this study, a recombinant hemolymph plasma lectin (rHPL(OE)) cloned from Taiwanese Tachypleus tridentatus was expressed in an Escherichia coli system. This rHPL(OE) was shown to have the following properties: (1) Binding to P. aeruginosa PA14 biofilm through a unique molecular interaction with rhamnose-containing moieties on bacteria, leading to reduction of extracellular di-rhamnolipid (a biofilm regulator); (2) decreasing downstream quorum sensing factors, and inhibiting biofilm formation; (3) dispersing the mature biofilm of P. aeruginosa PA14 to improve the efficacies of antibiotics; (4) reducing P. aeruginosa PA14 cytotoxicity to human lung epithelial cells in vitro and (5) inhibiting P. aeruginosa PA14 infection of zebrafish embryos in vivo. Taken together, rHPL(OE) serves as an anti-biofilm agent with a novel mechanism of recognizing rhamnose moieties in lipopolysaccharides, di-rhamnolipid and structural polysaccharides (Psl) in biofilms. Thus rHPL(OE) links glycan-recognition to novel anti-biofilm strategies against pathogenic bacteria.
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