期刊
LASERS IN SURGERY AND MEDICINE
卷 52, 期 4, 页码 358-372出版社
WILEY
DOI: 10.1002/lsm.23137
关键词
blue light; fibrosis; photobiomodulation; low-level light therapy; fibroblast; proteomics; phosphoproteomics
资金
- Taiwan Bio-Development Foundation (TBF)
- Taiwan Ministry of Science and Technology [104-2314-B-002-004-MY3, 106-2627-M-002-034, 107-2314-B-002-064-MY3]
- National Taiwan University Hospital [106-S3010, 107-S3781]
Background and Objectives This study was aimed at determining the effects of blue light photobiomodulation on primary adult mouse dermal fibroblasts (AMDFs) and the associated signaling pathways. Study Design/Materials and Methods Cultured AMDFs from adult C57BL/6 mice were irradiated by blue light from a light-emitting diode (wavelength = 463 +/- 50 nm; irradiance = 5 mW/cm(2); energy density = 4-8 J/cm(2)). The cells were analyzed using mass spectrometry for proteomics/phosphoproteomics, AlamarBlue assay for mitochondrial activity, time-lapse video for cell migration, quantitative polymerase chain reaction for gene expression, and immunofluorescence for protein expression. Results Proteomic/phosphoproteomic analysis showed inhibition of extracellular signal-regulated kinases/mammalian target of rapamycin and casein kinase 2 pathways, cell motility-related networks, and multiple metabolic processes, including carbon metabolism, biosynthesis of amino acid, glycolysis/gluconeogenesis, and the pentose phosphate pathway. Functional analysis demonstrated inhibition of mitochondrial activities, cell migration, and mitosis. Expression of growth promoting insulin-like growth factor 1 and fibrosis-related genes, including transforming growth factor beta 1 (TGF beta 1) and collagen type 1 2 chain diminished. Protein expression of alpha-smooth muscle actin, an important regulator of myofibroblast functions, was also suppressed. Conclusions Low-level blue light exerted suppressive effects on AMDFs, including suppression of mitochondrial activity, metabolism, cell motility, proliferation, TGF beta 1 levels, and collagen I production. Low-level blue light can be a potential treatment for the prevention and reduction of tissue fibrosis, such as hypertrophic scar and keloids. Lasers Surg. Med. (c) 2019 Wiley Periodicals, Inc.
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