期刊
LABORATORY ANIMALS
卷 53, 期 6, 页码 630-633出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0023677219856918
关键词
3Rs; ethics and welfare; genetics; organisms and models; rodents; experimental design; techniques; laboratory animal welfare
资金
- BBSRC [BB/R017220/1] Funding Source: UKRI
- MRC [MR/T002107/1] Funding Source: UKRI
- British Heart Foundation [RE/13/1/30181, FS/17/55/33100] Funding Source: Medline
- Medical Research Council [MR/T002107/1] Funding Source: Medline
Since it was introduced 20 years ago, tamoxifen-inducible genetic recombination in vivo has become a standard tool in many fields. This technique has great utility, allowing precise temporal and spatial gene recombination mediated by expression of a Cre recombinase-oestrogen receptor hormone binding domain fusion protein. It is frequently used in developmental biology, either for accurate spatio-temporal gene deletion or for lineage-labelling. Administration of high doses of tamoxifen can rapidly induce abortion in pregnant mice but this can be partially overcome by progesterone co-administration. However, administration of tamoxifen to pregnant mice early in pregnancy may have potentially lethal effects on the mother independently of abortion, and can also severely perturb embryonic development. Despite this, only a few published studies mention this fact in passing, and standard parameters for successful or unsuccessful use of tamoxifen in pregnant mice have not been reported. Therefore, in the interests of providing a framework for more humane animal research, we describe our experiences of tamoxifen administration during early gestation in mice. These observations should assist the design of future studies in accordance with the principles of the three Rs (Replacement, Reduction and Refinement of Animals in Research).
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