期刊
APPLIED SURFACE SCIENCE
卷 360, 期 -, 页码 28-36出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2015.10.178
关键词
Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM
类别
资金
- BioFibPlas project [35GA5652]
- SoMoPro II Programme
- People Programme (Marie Curie Action) of the Seventh Framework Programme of EU [291782]
- South-Moravian Region
- projects CEITEC - Central European Institute of Technology [CZ.1.05/1.1.00/02.0068]
- European Regional Development Fund
- Seventh Framework Programme of EU
- project Materials Research Centre at FCH BUT - Sustainability and Development [REG L01211]
- Structural Analysis Laboratory of CEITEC - Central European Institute of Technology under CEITEC - open access project [LM2011020]
- Ministry of Education, Youth and Sports of the Czech Republic [IZ73Z0_152661]
- Swiss National Science Foundation
The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据