期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 141, 期 27, 页码 10581-10584出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.9b02580
关键词
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资金
- Nanyang Technological University [NTU-SUG: M4081627]
- Singapore Ministry of Education Academic Research Fund Tier 1 [2017-T1-002-134-RG147/17]
- Singapore Ministry of Education Academic Research Fund Tier 2 [MOE2016-T2-1-098, MOE2018-T2-2-042]
- Tier 2
Real-time multiplex imaging is imperative to biology and diagnosis but remains challenging for optical modality. Herein, a unimolecular chemo-fluoro-luminescent reporter (CFR) is synthesized for duplex imaging of drug-induced hepatotoxicity (DIH), a long-term medical concern. CFR simultaneously detects superoxide anion (O-2(center dot)) and caspase-3 (casp3) through respective activation of its independent chemiluminescence and near-infrared fluorescence channels. Such a crosstalk-free duplex imaging capability of CFR enables longitudinal measurement of two correlated biomolecular events (oxidative stress and cellular apoptosis) during the progression of DIH, identifying O-2(center dot) as an earlier biomarker for detection of DIH both in vitro and in vivo. Moreover, CFR detects DIH 17.5 h earlier than histological changes. Thus, our study not only develops a sensitive optical reporter for early detection of DIH but also provides a general molecular design strategy for duplex imaging.
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