期刊
JOURNAL OF MOLECULAR NEUROSCIENCE
卷 69, 期 3, 页码 411-418出版社
HUMANA PRESS INC
DOI: 10.1007/s12031-019-01370-4
关键词
NLRP6; Cerebral ischemia/reperfusion injury; Inflammation; IL (interleukin)-1 beta; IL (interleukin)-18
资金
- National Natural Science Foundation of China [81671158, 81771261]
- National Youth Foundation of China [81701165]
- Natural Science Foundation of Chongqing Science and Technology Committee, China
The NOD-like receptor protein 6 (NLRP6), an intracytoplasmic pattern recognition receptor in the nucleotide-binding domain, leucine-rich repeat-containing (NLR) innate immune receptor family, influences the inflammation reaction. The role of NLRP6 in cerebral ischemia-reperfusion (I/R) injury in rats is unclear. We explore the function of NLRP6 in cerebral I/R injury. The investigators used a middle cerebral artery occlusion/reperfusion model (MCAO) to imitate ischemic injury. We found the peak expression of NLRP6 is in 48-h post-cerebral I/R injury. The expression of NLRP6 siRNA, as well as the expression of protein and mRNA, was detected by Western blot and qRT-PCR. The degree of IL-1 beta and IL-18 was assessed by ELISA. After downregulating NLRP6, the expression of IL-1 beta, IL-18, cleaved Caspase-1, and myeloperoxidase (MPO) were reduced. In HE and Nissl staining, pathological injury of brain tissue after downregulating NLRP6 was improved. NLRP6 siRNA decreased the NLRP6-ASC binding states by CO-IP. NRP6 has a pro-inflammatory effect in cerebral I/R injury, which may provide a new target for the treatment of cerebral I/R injury.
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