期刊
JOURNAL OF INFECTIOUS DISEASES
卷 220, 期 5, 页码 781-791出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiz208
关键词
human cytomegalovirus; genome sequence; target enrichment; genotype; variation; multiple-strain infection; recombination; gene loss; mutation
资金
- Medical Research Council [MC_UU_12014/3, MC_ UU_12014/12]
- Wellcome Trust [204870/Z/16/Z, WT090323MA]
- Ministry of Health of the Czech Republic (University Hospital, Motol, Prague, Czech Republic) [00064203]
- Fondazione Regionale per la Ricerca Biomedica, Regione Lombardia [FRRB 2015-043]
- Niedersachsische Ministerium fur Wissenschaft und Kultur (grant COALITION-Communities Allied in Infection)
- Deutsche Forschungsgemeinschaft Collaborative Research Centre 900 [SFB-9001]
- German Center of Infection Research Thematic Translational Unit Infections of the Immunocompromised Host
- Infection Biology graduate program of Hannover Biomedical Research School
- MRC [MC_UU_12014/12, MC_UU_12014/3] Funding Source: UKRI
The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.
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