期刊
JOURNAL OF IMMUNOLOGY
卷 203, 期 5, 页码 1276-1287出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1900377
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- Bloodwise UK [15021, 100326Z/12/Z]
CD4(+) T cells are essential for immune protection against viruses, yet their multiple roles remain ill-defined at the single-cell level in humans. Using HLA class II tetramers, we studied the functional properties and clonotypic architecture of EBV-specific CD4(+) T cells in patients with infectious mononucleosis, a symptomatic manifestation of primary EBV infection, and in long-term healthy carriers of EBV. We found that primary infection elicited oligoclonal expansions of T(H)1-like EBV-specific CD4(+) T cells armed with cytotoxic proteins that responded immediately ex vivo to challenge with EBV-infected B cells. Importantly, these acutely generated cytotoxic CD4(+) T cells were highly activated and transcriptionally distinct from classically described cytotoxic CD4(+) memory T cells that accumulate during other persistent viral infections, including CMV and HIV. In contrast, EBV-specific memory CD4(+) T cells displayed increased cytokine polyfunctionality but lacked cytotoxic activity. These findings suggested an important effector role for acutely generated cytotoxic CD4(+) T cells that could potentially be harnessed to improve the efficacy of vaccines against EBV.
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