期刊
JOURNAL OF HOSPITAL INFECTION
卷 103, 期 1, 页码 78-84出版社
W B SAUNDERS CO LTD
DOI: 10.1016/j.jhin.2019.04.011
关键词
Clostridium difficile; Sporicidal activity; Reproducibility; Peracetic acid; Glutaraldehyde
资金
- Association for Applied Hygiene (VAH), Bonn, Germany
Background: Sporicidal surface disinfection is recommended to control transmission of Clostridium difficile in healthcare facilities. EN 17126 provides a method to determine the sporicidal activity in suspension and has been approved as a European standard. In addition, a sporicidal surface test has been proposed. Aim: To determine the interlaboratory reproducibility of a test method for evaluating the susceptibility of a C. difficile spore preparation to a biocidal formulation following the 4-field test (EN 16615 methodology). Methods: Nine laboratories participated. C. difficile NCTC 13366 spores were used. Glutaraldehyde (1% and 6%; 15 min) and peracetic acid (PAA; 0.01% and 0.04%; 15 min) were used to determine the spores' susceptibility in suspension in triplicate. Findings: One-percent glutaraldehyde revealed a mean decimal log(10) reduction of 1.03 with variable results in the nine laboratories (0.37-1.49) and a reproducibility of 0.38. The effect of 6% glutaraldehyde was stronger (mean: 2.05; range: 0.96-4.29; reproducibility: 0.86). PAA revealed similar results. An exemplary biocidal formulation based on 5% PAA was used at 0.5% (non-effective concentration) and 4% (effective concentration) to determine the sporicidal efficacy (4-field test) under clean conditions in triplicate with a contact time of 15 min. When used at 0.5% it demonstrated an overall log(10) reduction of 2.68 (range: 2.35-3.57) and at 4% of 4.61 (range: 3.82-5.71). The residual contamination on the three primarily uncontaminated test fields was <50 cfu/25 cm(2) in one out of nine laboratories (0.5%) and in seven out of nine laboratories (4%). Conclusion: The interlaboratory reproducibility seems to be robust. (C) 2019 The Authors. Published by Elsevier Ltd on behalf of The Healthcare Infection Society.
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