期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 374, 期 -, 页码 203-210出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2019.04.027
关键词
Aquatic plant; Chiral capillary electrophoresis; Duloxetine; Econazole; Mixture toxicity and stability
资金
- Ministry of Economy and Competitiveness (Spain) [CTQ2013-48740-P, CTQ2016-76368-P, BES-2014-070532]
- Direccion General de Universidades e Investigacion de la Comunidad de Madrid (Spain) [S2013/MAE-2716]
- ICTS NANBIOSIS, Confocal Microscopy Service: Giber in Bioengineering, Biomaterials AMP
- Nanomedicine (CIBER-BNN) at the Alcala University (CAI Medicine Biology)
- University of Alcala
Stability and toxicity studies for duloxetine and econazole were achieved using individual solutions and their mixtures. Stability of drugs racemates and enantiomers was investigated under abiotic and biotic conditions. Toxicity was evaluated for the first time on Spirodela polyrhiza. EC50 values were calculated for each individual drug and for their binary mixture. Real (not nominal) concentrations determined by Capillary Electrophoresis were employed in the calculations of toxicity parameters. The use of a 25 mM phosphate buffer (pH 3.0) with 1.5% S-beta-CD as chiral selector at a temperature of 30 degrees C and a separation voltage of - 20 kV enabled the simultaneous enantiomeric separation of duloxetine and econazole in 7.5 min with enantiomeric resolutions of 7.9 and 6.5, respectively. For individual solutions, decay percentages under abiotic conditions were higher for duloxetine (80%) than for econazole (60%), while in presence of Spirodela polyrhiza they increased for duloxetine but not for econazole. Econazole showed the highest decay percentages under abiotic or biotic conditions (100%) in binary mixtures. EC50 values for duloxetine and econazole enabled to include both drugs within the group of very toxic compounds although econazole showed a higher toxicity than duloxetine and the binary mixture.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据