期刊
JOURNAL OF FOOD AND DRUG ANALYSIS
卷 27, 期 4, 页码 906-914出版社
FOOD & DRUG ADMINSTRATION
DOI: 10.1016/j.jfda.2019.06.001
关键词
Cardiovascular drugs; Dried blood spot; LC-MS/MS; Therapeutic drug monitoring
资金
- Bio-Synergy Research Project of the Ministry of Science, ICT and Future Planning through the National Research Foundation [NRF-2012M3A9C4048796]
- National Research Foundation of Korea - Korean government (MSIP) [NRF-2018R1A5A2024425]
A dried blood spot (DBS) sampling method was exploited to extract cardiovascular drugs using a small volume of whole blood of human and rodent. Thereafter, an analytical method using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of 12 cardiovascular drugs. A 6 mm internal diameter disc containing 10 mu L of blood was punched from a specifically designed card and analyzed by LC-MS/MS using a gradient elution method with a total run time of 16 min. For sample separation, a universal octadecyl-silica column was used with a flow rate of 0.2 mL/min. The developed method was validated in terms of linearity, accuracy, and precision, which showed satisfactory results. In addition, the matrix effects were closely investigated to confirm the extraction efficiency. Additionally, the stability was tested by storing DBSs at room temperature; the results showed that these drugs were stable for at least 30 days. Accordingly, the proposed LC-MS/MS method is capable to analyze several cardiovascular drugs in a single analysis. It can be applied to therapeutic drug monitoring in patients as well as in the in vivo settings. Copyright (C) 2019, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据