期刊
JOURNAL OF FISH DISEASES
卷 42, 期 9, 页码 1301-1307出版社
WILEY
DOI: 10.1111/jfd.13052
关键词
detection; faeces; intragastric challenge; tilapia; tilapia lake virus
资金
- University of California, School of Veterinary Medicine
To gain a better understanding of the pathogenesis of tilapia lake virus (TiLV) infections in Nile tilapia (Oreochromis niloticus), fingerlings were challenged with a single dose of 1 x 10(4) TCID50/fish of TiLV utilizing intracoelomic/intraperitoneal (ICch) or intragastric (IG(ch)) routes. Acute mortalities were present in both groups, reaching 70 and 40% in ICch and IG(ch) after 10 days, respectively. Challenged fish presented erratic swimming, lethargy, anorexia, exophthalmia and cutaneous petechiae and ecchymoses. Histological changes in challenged groups included syncytial formation, intracytoplasmic inclusion bodies and multifocal hepatocellular degeneration and necrosis. In addition, multifocal areas of mild proliferation of glial cells and lymphocytic perivascular cuffing were observed in the brain of exposed challenged groups. TiLV RNA was detected in gills and faeces of challenged fish using quantitative reverse transcriptase-PCR, as well as in the tank water holding challenged fish. Moreover, TiLV RNA was detected in scrolls obtained from formalin-fixed paraffin-embedded tissue blocks from challenged fish. Results from this study suggest that IG methods represent an additional method to study the pathogenesis of the disease in this species, as it results in infection and diseases as in naturally occurring cases and does not bypass important mucosal immune responses as injectable routes do.
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