期刊
BIOMEDICAL MICRODEVICES
卷 17, 期 6, 页码 -出版社
SPRINGER
DOI: 10.1007/s10544-015-0019-x
关键词
Microfluidic tissue culture; Retina; Microenvironment
资金
- National Eye Institute [RO1EY020496-01, P30EY08126]
- Research to Prevent Blindness, Inc.
- National Science Foundation [0909667, 1445197]
- Division Of Graduate Education
- Direct For Education and Human Resources [0909667] Funding Source: National Science Foundation
We report on a microfluidic platform for culture of whole organs or tissue slices with the capability of point access reagent delivery to probe the transport of signaling events. Whole mice retina were maintained for multiple days with negative pressure applied to tightly but gently bind the bottom of the retina to a thin poly-(dimethylsiloxane) membrane, through which twelve 100 mu m diameter through-holes served as fluidic access points. Staining with toluidine blue, transport of locally applied cholera toxin beta, and transient response to lipopolysaccharide in the retina demonstrated the capability of the microfluidic platform. The point access fluidic delivery capability could enable new assays in the study of various kinds of excised tissues, including retina.
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