期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 23, 期 9, 页码 6140-6153出版社
WILEY
DOI: 10.1111/jcmm.14490
关键词
BMSCs; ICAM-1; melatonin; MiRNA; osteogenic differentiation; osteoporosis
资金
- National Natural Science Foundation of China [81501920, 81573434, 81800784]
- Postdoctoral Science Foundation of China [2018M630370]
- Heilongjiang Postdoctoral Fund [LBH-Z17134]
- Natural Science Foundation of Heilongjiang Province [LC2015027, H2015056]
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2015034]
Osteoporosis is closely associated with the dysfunction of bone metabolism, which is caused by the imbalance between new bone formation and bone resorption. Osteogenic differentiation plays a vital role in maintaining the balance of bone microenvironment. The present study investigated whether melatonin participated in the osteogenic commitment of bone marrow mesenchymal stem cells (BMSCs) and further explored its underlying mechanisms. Our data showed that melatonin exhibited the capacity of regulating osteogenic differentiation of BMSCs, which was blocked by its membrane receptor inhibitor luzindole. Further study demonstrated that the expression of miR-92b-5p was up-regulated in BMSCs after administration of melatonin, and transfection of miR-92b-5p accelerated osteogenesis of BMSCs. In contrast, silence of miR-92b-5p inhibited the osteogenesis of BMSCs. The increase in osteoblast differentiation of BMSCs caused by melatonin was attenuated by miR-92b-5p AMO as well. Luciferase reporter assay, real-time qPCR analysis and western blot analysis confirmed that miR-92b-5p was involved in osteogenesis by directly targeting intracellular adhesion molecule-1 (ICAM-1). Melatonin improved the expression of miR-92b-5p, which could regulate the differentiation of BMSCs into osteoblasts by targeting ICAM-1. This study provided novel methods for treating osteoporosis.
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