期刊
JOURNAL OF CATALYSIS
卷 374, 期 -, 页码 306-319出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcat.2019.05.006
关键词
Deep eutectic solvent; Polymer; Enzyme; Adsorption; Hydrolysis
资金
- Natural Science Foundation of Hebei Province [E2018201155, B2018201270]
- Open Project Fund of Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education [NRFM201705]
- Creation and Innovation Program of Hebei Province University Students [201810075085]
- High-level Talents Introduction Program of Hebei University
- National Natural Science Foundation of China [21575033, 81803287]
Biocompatible and magnetic polymers of poly(vinyl pyrrolidone deep eutectic solvent, VP DES) were investigated to determine whether they could efficiently adsorb L-asparaginase, which is an important enzyme inhibiting the growth of certain tumor cells. A series of poly(VP DES)s were successfully obtained, and that based on VP-malonic acid (VP/MA molar ratio of 1:1) was found to be the best for L-asparaginase adsorption. In addition, L-asparaginase was easily separated with biocompatible poly (VP-MA DES) from Escherichia coli extracts under a magnetic field with a high specific activity toward the hydrolysis of L-asparagine, as determined by the Nessler reaction method. Based on this work, poly(VP-MA DES)s seem ideal carriers via adsorption for the delivery of specific enzyme for the biocatalysis. (C) 2019 Elsevier Inc. All rights reserved.
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