期刊
JOURNAL OF BIOPHOTONICS
卷 12, 期 12, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201900177
关键词
biofluids; FTIR spectroscopy; plasma; pre-analytical requirements; standardisation; serum; water interference correction
资金
- Defence and Science Technology Laboratory (Dstl, United Kingdom)
- Direction Generale de l'Armement (DGA, France)
- Societe d'Acceleration du Transfert de Technologie (SATT) NORD
Infrared spectroscopy is a rapid, easy-to-operate, label-free and therefore cost-effective technique. Many studies performed on biofluids (eg, serum, plasma, urine, sputum, bile and cerebrospinal fluid) have demonstrated its promising application as a clinical diagnostic tool. Given all these characteristics, infrared spectroscopy appears to be an ideal candidate to be implemented into the clinics. However, before considering its translation, a clear effort is needed to standardise protocols for biofluid spectroscopic analysis. To reach this goal, careful investigations to identify and track errors that can occur during the pre-analytical phase is a crucial step. Here, we report for the first time, results of investigations into pre-analytical factors that can affect the quality of the spectral data acquired on serum and plasma, such as the impact of long-term freezing time storage of samples as well as the month-to-month reproducibility of the spectroscopic analysis. The spectral data discrimination has revealed to be majorly impacted by a residual water content variation in serum and plasma dried samples.
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