期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 26, 页码 7378-7389出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b02759
关键词
juglone; Carya cathayensis Sarg; Ishikawa; cell cycle; apoptosis; reactive oxygen species
资金
- Major Projects of Science and Technology in Yunnan Province [2018ZG004]
- National Natural Science Foundation of China [31850410476]
- Major Projects of Science and Technology in Anhui Province [1804b06020347, 17030701028, 18030701142, 18030701158]
The molecular mechanism of Juglone-induced cell cycle arrest and apoptosis in human endometrial cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 mu M, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein (p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, CHK1, and cyclin A. The expression of Bcl-2 and Bc1-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural anticancer agent.
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