期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
卷 55, 期 1, 页码 267-275出版社
WILEY
DOI: 10.1111/ijfs.14302
关键词
Anti-listerial synergistic activity; bacteriocins; calcium propionate; lactocin AL705; nisin; potassium sorbate; sodium lactate
资金
- INTI [710/14]
- MINCyT-ANPCyT [PICT-2014-0585, PICT-2016-0407]
The effectiveness of antimicrobial mixtures against Listeria innocua 7, used as a L. monocytogenes surrogate, was investigated in broth and a food system. Synergistic effects were found for nisin (Nis), potassium sorbate (PS), calcium propionate (CP) and sodium lactate (SL), Nis + PS being the most effective binary mixture that exhibited listericidal activity in broth. To assess the effect of adding lactocin AL705 (AL705) to Nis + organic acid salt combinations, tridimensional isobolograms were generated. Sub-MIC combinations of the antimicrobials exerted bactericidal activity against L. innocua 7 after AL705 addition to the binary mixtures. However, when applied on Sardo cheese contaminated with L. innocua 7 (initial inoculum 4.45 +/- 0.06 CFU g(-1)), only Nis + PS + AL705 produced count reductions respect to the control, reaching 3.04 +/- 0.35 CFU g(-1) counts after 15 days at 15 degrees C. Ternary combinations containing AL705 showed potential to reduce antimicrobial usages for L. innocua 7 inhibition.
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