期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 132, 期 -, 页码 801-810出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.04.015
关键词
Polysaccharide-iron complex; Flammulina velutipes; Caco-2 cells; Cell cytotoxicity; Transport mechanism
资金
- State key research and development plan Modern food processing and food storage and transportation technology and equipment [2017YFD0400203]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX18_0755]
Iron is essential in fundamental bioactivities, so it makes sense to improve the efficiency of iron on epithelial transport. In this work, a novel polysaccharide-iron(III) complex (FVP-Fe(III)) was prepared from Flammulina velutipes with a specific structure. The FVP-Fe(III) had a molecular weight of 15.13 kDa with a monosaccharide composition of mannose, galacturonic acid, glucose, galactose, xylose, fucose in a molar ratio 3.6:2.1:60.8:18.7:3.9:10.9. In the vitro digestion model, the complex could maintain better solubility and steady of iron than FeSO4. In the cell assay, FVP-Fe(Ill) showed lower cytotoxicity and better absorption. The transport amount of FVP-Fe(III) was 1.5-fold of FeSO4 at same concentration and 1.8-fold of FeSO4 at same time. The transport was mediated by the peptide transporter (pepT1) active transport pathway and the efflux of the sample was mainly mediated by multidrug-resistance proteins (MRP) transporter. The results of this study suggested that the polysaccharide obtained from F. velutipes could be developed a new kind of iron delivery for further study. (C) 2019 Elsevier B.V. All rights reserved.
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