期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 130, 期 -, 页码 564-572出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.02.163
关键词
Graphene oxide nanosheets; Penaeus vannamei protease; Enzyme immobilization; Enzyme stabilization; Glutaraldehyde
资金
- Iran National Science Foundation (INSF) [96000161]
In this study, we report the synthesis of graphene oxide nanosheets (GON) by a modified Hummers method. Then, a protease purified from the Penaeus vannamei shrimp was immobilized on the GON activated with glutaraldehyde. Several techniques such as SEM, DLS and FTIR were applied to characterize the different nano structures at the different levels. The immobilization of the protease on the GON activated with glutaraldehyde did not affect the optimum pH, but significantly improved thermal stability and stability at extreme pH values, as well as activity at 90 degrees C. After 24 h of incubation at 90 degrees C, the free enzyme retained less than 10% of the activity, while the immobilized enzyme kept more than 90% of its original activity. The apparent K-m and V-max for Penaeus vannamei protease remained fairly similar after immobilization, a very relevant data considering the large size of the substrate (casein). In the hydrolysis of casein at 70 degrees C and in the presence of 2 M urea, the immobilized enzyme exhibited a higher activity than the free enzyme. The results indicate that the immobilization of the enzyme Penaeus vannamei protease on GON activated with glutaraldehyde increases its already high stability against environmental stresses and makes it suitable for biotechnological and industrial applications. (C) 2019 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据