期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 133, 期 -, 页码 306-315出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.04.082
关键词
Lilium davidii var. unicolor Cotton; Heteropolysaccharides; Antioxidant and antibacterial activity; Structural characterization
资金
- National Key Research and Development Program of China [2017YFD0200804]
- National Natural Science Foundation of China [31570354, 21775154, 31772668, 21302195, 31560037]
- CAS Light of West China Program
Two heteropolysaccharides, BHP-1 and BHP-2, were separated and purified from the edible bulbs of Lilium davidii var. unicolor Cotton by anion-exchange and gel-permeation chromatography. The primary structural characteristics of BHP-1 and BHP-2 were investigated by Congo red test, X-ray diffractometry (XRD) analysis, IR, GC and NMR. The results showed that the weight-average molecular weight (Mw) and number-average molecular weight (Mn) of BHP-1 and BHP-2 were nearly 1.93 x 105 g/moL, 8.86 x 104 g/moL and 3.52 x 104 g/moL, 2.95 x 104 g/moL, respectively. Glucose and mannose comprised BHP-1 in a relative molar ratio of 5.9:2.0, and BHP-2 was consisted of glucose, galactose, mannose and arabinose with approximate molar ratios of 8.3:1.5:1.0:1.1. Their backbone mainly contained (x-(1 > 4)-linked o-glucopyranosyl. In addition, the C-6 of the glucose and C-2 of mannose residue of BHP-1 and BHP-2 were substituted. The two polysaccharides were semi-crystalline substance with multi-branching structure and triple helix conformation. Bioassays showed that BHP-1 and BHP-2 had effective antioxidant activities evaluated by DPPH, scavenging superoxide radical and OH radical in a dose-dependent manner. The two heteropolysaccharides also displayed distinct and selective antibacterial activity against B. cereus, P. putida, M. luteus and K. pneumonia with inhibition zones of 13 mm, 16 mm, 17 mm, 23 mm and 12 mm, 17 mm, 19 mm, 20 mm, respectively. These findings provided a reference for potential applications of the polysaccharides from L davidii as natural antioxidants and antibacterial agents in food and pharmaceuticals. (C) 2019 Elsevier B.V. All rights reserved.
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