期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 133, 期 -, 页码 1029-1034出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.04.114
关键词
Antarctic krill; Chitin; Chitinase; N-acetyl-D-glucosamine; Protease
资金
- National Key Research and Development Program of China [2017YFD0200500]
- National Natural Science Foundation of China [31871959]
Defatted krill powder (DKP), the byproduct of krill oil industry, is a resource of biological macromolecules. Here, one bacterial protease, three bacterial chitinases and one insect N-acetyl-D-hexosaminidase were integratively used to produce peptide, N,N'-diacetylchitobiose [(GlcNAc)(2)] and N-acetyl-D-glucosamine (GlcNAc) from DKP. First, alkaline protease was found to outperform neutral protease in deproteinizing DKP and the resultant krill peptides were rich in essential amino acids (41.4%). Second, the mutant of chitinase A from Serratia marcescens [SmChiA-F232W/F396W (SmChiA-M)] was found to be 32% faster than wild-type SmChiA in hydrolyzing the deproteinized DKP (DDKP) and showed significant synergy with chitinase B from S. marcescens (SmChiB) and chitinase C from S. marcescens (SmChiC). Then two SmChiA-M-based enzyme combinations [SmChiA-M + SmChiB + SmChiC and SmChiA-M + SmChiB + SmChiC + OfHex1 (an insect N-acetyl-D-hexosaminidase from Ostrinia furnacalis)] were designed to produce (GlcNAc)(2) and GlcNAc, respectively, from DDKP. A yield of 2.04 g/L (GlcNAc)(2) or 2.71 g/L GlcNAc (each with 95% purity) could be obtained from 20 g/L DDKP in 24 h. (C) 2019 Elsevier B.V. All rights reserved.
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